By Alan S. Curry
This number of 9 essays by way of best specialists considers the applying of the main lately constructed analytical recommendations and gear to specific poisons and medicine. It describes using HPLC, fluorescence research, and radioimmunoassays in toxicology and indicates equipment particular to forensic medication.
Read or Download Analytical Methods in Human Toxicology: Part 2 PDF
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Additional resources for Analytical Methods in Human Toxicology: Part 2
Since there is no cheap and sensitive universal method for detecting all types of compounds which may be separated by HPLC, the detector selected for use will depend upon the properties of the compounds to be analysed . ) spectra, with the outcome that the most frequently encountered detector in toxicological applications is the ultraviolet spectrophotometer. A vast range of ultraviolet spect rophotometers is available , ranging from fixed wavelength instruments offering sample measurement at one single predetermined wavelength to sophisticated variablewavelength instruments.
Moffat, A. C. (1974). , and O'Connor, M. ). Drugs and the Poisoned Patient - The Role of the Laboratory, Ciba Foundation Symposium No. 26. Elsevier, Amsterdam and Oxford, 83-100. Mrochek , J. , Christe, W. , and Dinsmore, S. R. (1974). cu« Chem. 20, 1086-96. Nation, R. , Peng, G. , and Chiou , W. L. (1978). J. Pharm. Sci. 67 , 805-8. Osselton, M. D. (1981). In Reid, E. ). Trace Organic Sample Handling, Vol. 10, Methodological Surveys in Biochemistry. Ellis Horwood, Chichester, 10 1-10. Paanakker, J .
1976), or use of reagents of high ionic strength. A related technique (particularly useful with tissue samples) is digestion of the biological material by proteolytic enzymes (Osselton, 1981). In many tissues the chemical of interest may be wholly or partly present as a conjugate (phase 2 metabolite) such as a glucuronic acid, sulphate or glutathione derivative ; these are seldom amenable to solvent extraction . g. , 1974) where conjugates have been chromatographed directly by HPLC, it is more common to hydrolyse them , either enzymatically or using acid .